Article

Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR-Cas13d in mammalian cells

2021-12-21

Abstract excerpt

Cas13 is a unique family of CRISPR endonucleases exhibiting programmable binding and cleavage of RNAs and is a strong candidate for eukaryotic RNA knockdown in the laboratory and the clinic. However, sequence-specific binding of Cas13 to the target RNA unleashes non-specific bystander RNA cleavage, or collateral activity, which may confound knockdown experiments and raises concerns for therapeutic applications. Al...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7c67b42f-4737-5ea7-af92-65bd92cf7b05
DOI: 10.1101/2021.12.20.473384

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR-Cas13d in mammalian cellsDOI 10.1101/2021.12.20.473384Read

High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effectSemantic proximity Read

Target RNA abundance controls the collateral activity of RfxCas13d in human cells and zebrafish embryosSemantic proximity Read

Collateral cleavage of 28s rRNA by RfxCas13d causes death of miceSemantic proximity Read

Optimizing a CRISPR-Cas13d gene circuit for tunable target RNA downregulation with minimal collateral RNA cuttingSemantic proximity Read

Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cellsSemantic proximity Read

The sustained expression of Cas9 targeting toxic RNAs reverses disease phenotypes in mouse models of myotonic dystrophy type 1.Semantic proximity Read

Precise transcript targeting by CRISPR-Csm complexesSemantic proximity Read

Engineered CRISPR RNA improves the RNA cleavage efficiency of hfCas13X.Semantic proximity Read

Principles for rational Cas13d guide designSemantic proximity Read

Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targetingSemantic proximity Read

Robust CRISPR/dCas13 RNA blockers specifically perturb miRNA-target interactions and rescue type 1 myotonic dystrophy pathologySemantic proximity Read

Targeting Toxic Nuclear RNA Foci with CRISPR-Cas13 to Treat Myotonic DystrophySemantic proximity Read