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Article

Engineered CRISPR RNA improves the RNA cleavage efficiency of hfCas13X.

FEBS letters - 1 Oct 2024

Liu Zehui, Zhang Wenxia, Wang Haili, Shangguan Pingping, Pan Tong, Yang Yimu, Zhang Yi, Mao Xi, Liu Yingle, Zhang Qi

Abstract excerpt

As the most compact variant in the Cas13 family, CRISPR-Cas13X holds considerable promise for gene therapy applications. The development of high-fidelity Cas13X (hfCas13X) mutants has enhanced the safety profile for in vivo applications. However, a notable reduction in on-target cleavage efficiency accompanies the diminished collateral cleavage activity in hfCas13X. In this study, we obtained two engineered crRNA...

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Identifiers

PMID: 39327223
DOI: 10.1002/1873-3468.15025

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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