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Article

RNA target-independent non-canonical activation (RINCA) of Cas13 trans-nuclease activity.

Science bulletin - 30 Sept 2025

Liu Wenjun, Zhu Xuena, Xue Yuanyuan, Wang Xiaoyan, Chang Lei, Wang Shanshan, Li Ling, Huang Jinyan, Zhang Ying, Jiang Cheng, Chi Hongjie, Zhang Yanbin, Li Chen-Zhong, Liang Tingbo

Abstract excerpt

A thorough understanding of unintended Cas13 activity is critical for ensuring the safe in vivo application of CRISPR-Cas13. In this study, we uncover the RNA target-independent, non-canonical activation (RINCA) of Cas13 by crRNA alone and elucidate its structural basis. RINCA poses both challenges and opportunities for Cas13-based technologies. On one hand, it constrains Cas13's utility due to its uncontrolled...

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Identifiers

PMID: 40769810
DOI: 10.1016/j.scib.2025.07.015

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