Article
Optimized strategy for in vivo Cas9-activation in Drosophila.
Proceedings of the National Academy of Sciences of the United States of America - 29 Aug 2017
Ewen-Campen Ben, Yang-Zhou Donghui, Fernandes Vitória R, González Delfina P, Liu Lu-Ping, Tao Rong, Ren Xingjie, Sun Jin, Hu Yanhui, Zirin Jonathan, Mohr Stephanie E, Ni Jian-Quan, Perrimon Norbert
Abstract excerpt
While several large-scale resources are available for in vivo loss-of-function studies in Drosophila, an analogous resource for overexpressing genes from their endogenous loci does not exist. We describe a strategy for generating such a resource using Cas9 transcriptional activators (CRISPRa). First, we compare a panel of CRISPRa approaches and demonstrate that, for in vivo studies, dCas9-VPR is the most optimal...
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