Article

Highly frequent undesired insertional mutagenesis during <i>Drosophila</i> genome editing

2025-08-11

Abstract excerpt

<h4>Summary</h4> CRISPR/Cas9 based genome editing employing Homology Directed Repair (HDR) from template vector sequences is a widely used technique to enable precise insertions, deletions or modifications to genes. Here, we describe an undesired and highly frequent editing event when using conventional CRISPR/Cas9 plus HDR methods for Drosophila melanogaster germline genome editing. We find that the template ve...

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Literature Corpus work: bde638ee-041e-5bc3-bd69-b18a9e603e42
DOI: 10.1101/2025.08.11.669658

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