Article

Highly efficient CRISPR/Cas9-mediated tissue specific mutagenesis in <i>Drosophila</i>

2018-02-20

Abstract excerpt

<h4>ABSTRACT</h4> Tissue-specific loss-of-function (LOF) analysis is an essential approach for characterizing gene function. Here we describe an efficient CRISPR -mediated t issue- r estricted m utagenesis (CRISPR-TRiM) method for ablating gene function in Drosophila . This binary system consists of a tissue-specific Cas9 and a ubiquitously expressed multi-guide RNA (gRNA) transgene. To facilitate the constr...

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Literature Corpus work: 263ab619-b016-5c55-b2df-ff3b382ae5fd
DOI: 10.1101/268482

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