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CRISPR-Cas9 repair complexity in <i>Drosophila melanogaster</i> : NHEJ-induced deletions and HDR variability in the <i>bantam</i> microRNA gene

2025-05-30

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas9–mediated gene editing was used to generate specific mutants of the bantam gene in Drosophila melanogaster . To drive non-homologous end joining (NHEJ) and achieve a precise deletion of most of the bantam locus, two guide RNAs targeting sites 90 base pairs apart were expressed in the germline using the UAS/GAL4 system. Thirty lethal and eight viable lines were established and anal...

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Literature Corpus work
c38a9478-7fea-5fdf-8b41-a8d6429885ea
DOI
10.1101/2025.05.29.656857
Open publication

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CRISPR-Cas9 repair complexity in <i>Drosophila melanogaster</i> : NHEJ-induced deletions and HDR variability in the <i>bantam</i> microRNA geneDOI 10.1101/2025.05.29.656857
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