Article

Pooled analysis of radiation hybrids identifies loci for growth and drug action in mammalian cells

2019-12-17

Abstract excerpt

Genetic screens in mammalian cells commonly focus on loss-of-function approaches. To evaluate the phenotypic consequences of extra gene copies, we used bulk segregant analysis (BSA) of radiation hybrid (RH) cells. We constructed six pools of RH cells, each consisting of ~2500 independent clones, and placed the pools under selection in media with or without paclitaxel. Low pass sequencing identified 859 growth loci...

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Literature Corpus work: db943391-7ccc-59bb-87ac-bfb881123d0b
DOI: 10.1101/2019.12.16.877365

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