Article
Survey sequencing and radiation hybrid mapping to construct comparative maps.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Hitte Christophe, Kirkness Ewen F, Ostrander Elaine A, Galibert Francis
Abstract excerpt
Radiation hybrid (RH) mapping has become one of the most well-established techniques for economically and efficiently navigating genomes of interest. The success of the technique relies on random chromosome breakage of a target genome, which is then captured by recipient cells missing a preselected marker. Selection for hybrid cells that have DNA fragments bearing the marker of choice, plus a random set of DNA...
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