Generating long deletions across the genome with pooled paired prime editing screens

Abstract excerpt

Engineered deletions are a powerful probe for studying genome architecture, function, and regulation. Yet, the lack of effective methods to create them in large numbers and at multi-kilobase scale has rendered interrogating all the gigabases of human DNA elusive. Prime editing based approaches are promising contenders to fill this gap, but have not been adopted for large-scale pooled screening so far. Here, we per...

Topics

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• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Embryo, Mammalian
• Gene Editing
• Genome
• Mice
• Polymorphism, Single Nucleotide
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

37a72c74-1346-53b9-8707-58e935e5b34c

DOI

10.1101/2025.11.03.686307