Genetic screens in isogenic mammalian cell lines without single cell cloning

Abstract excerpt

Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs for mammalian cells, however, is labor-intensive, time-consuming, and impossible in some cell types. Here we present an approach to create isogenic pairs of cells and screen them with genome-wide CRISPR-Cas9 libraries to generate genetic interaction maps. We queried...

Topics

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• Binding Sites
• Cell Line
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• Gene Editing
• HEK293 Cells
• MicroRNAs
• Mutation

Identifiers and source

Literature Corpus work

620e17bd-d973-52ad-a8dd-d8e62b1b9360

DOI

10.1101/677385