Article

Improving FnCas12a genome editing by exonuclease fusion

2020-07-08

Abstract excerpt

Among current reported Cas12a orthologs, Francisella novicida Cas12a (FnCas12a) is less restricted by protospacer adjacent motif (PAM), which will help target previously inaccessible genomic sites. However, the activity of FnCas12a nuclease is relatively low or undetectable in human cells, limiting its application as desirable genome engineering tools. Here, we describe TEXT ( T ethering EX onuclease T 5 with F...

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Literature Corpus work: f231dbcf-7c03-5187-a461-436691ca19bc
DOI: 10.1101/2020.07.07.191130

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