Article

PAM-Flexible Genome Editing with an Engineered Chimeric Cas9

2023-03-07

Abstract excerpt

<title>Abstract</title> <p>CRISPR enzymes require a defined protospacer adjacent motif (PAM) flanking a guide RNA-programmed target site, limiting their sequence accessibility for robust genome editing applications. In this study, we recombine the PAM-interacting domain of SpRY, a broad-targeting Cas9 possessing an NRN > NYN PAM preference, with the N-terminus of Sc++, a Cas9 with simultaneously broad, efficient,...

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Identifiers and source

Literature Corpus work: 274034aa-2723-598a-9e0a-d1a8f19eced8
DOI: 10.21203/rs.3.rs-2625838/v1

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