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CRISPR technologies and the search for the PAM-free nuclease

22 Jan 2021

Abstract excerpt

The ever-expanding set of CRISPR technologies and their programmable RNA-guided nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with an ever-present constraint: the requirement for a protospacer adjacent motif (PAM) flanking each target. While PAMs play an essential role in self/nonself discrimination by CRISPR-Cas immune systems, this constraint has launched a...

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PMID: 33483498
DOI: 10.1038/s41467-020-20633-y

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