Article

Tailored identification of Cas9 proteins with specific PAM requirements using a computational prediction pipeline

2022-05-20

Abstract excerpt

<title>Abstract</title> <p>The identification of the protospacer adjacent motif (PAM) sequences of Cas9 nucleases is crucial for their exploitation as genome editing tools. Here we interrogated a massively expanded dataset of metagenome and virome assemblies for accurate and comprehensive PAM predictions, in order to identify novel Cas9s selected for their PAM requirements. As a test bed we selectively isolated a...

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Literature Corpus work: 0a264434-4820-514c-995c-57ef26a81a6b
DOI: 10.21203/rs.3.rs-1652795/v1

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