Article

Expanding the CRISPR toolbox by engineering Cas12a orthologs of metagenomic discovery

2024-09-27

Abstract excerpt

Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility in genome editing and molecular diagnostic applications. However, the widespread adoption of CRISPR-Cas12a nucleases and their variants has been hindered by the requirement for a specific protospacer adjacent motif (PAM), relatively low CRISPR RNA (crRNA) activity and the inability to multiplex nucleic acid detection alone. To overcome these limitati...

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Literature Corpus work: 7c0b625c-ff5f-596b-b310-646b7b6152c9
DOI: 10.1101/2024.09.27.615316

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