Article

CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells

2021-07-01

Abstract excerpt

<h4>ABSTRACT</h4> The CRISPR/Cas9 system is widely used to permanently delete genomic regions by inducing double-strand breaks via dual guide RNAs. However, on-target consequences of Cas9 deletion events have yet to be fully investigated. To characterize Cas9-induced genotypic abnormalities in human cells, we utilized an innovative droplet-based target enrichment approach followed by long-read sequencing and coup...

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Literature Corpus work: 0bf17f97-b418-50e7-acb4-7c785b99790c
DOI: 10.1101/2021.07.01.450727

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