Article

Detection of deleterious on-target effects after HDR-mediated CRISPR editing

2020-03-29

Abstract excerpt

<h4>Summary</h4> CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on-target at the edited locus and off-target at other genomic loci. We investigated the occurrence of deleterious on-target effects in human stem cells after insertion of disease-related mutations by homology-directed repair (HDR). We identified large, mono-allelic genomic deletions...

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Literature Corpus work: d6290990-2d32-512f-b8d3-adea2b20c6ac
DOI: 10.1101/2020.03.27.012104

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