Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells.

Nature communications - 13 Aug 2021

Boutin J, Rosier J, Cappellen D, Prat F, Toutain J, Pennamen P, Bouron J, Rooryck C, Merlio J P, Lamrissi-Garcia I, Cullot G, Amintas S, Guyonnet-Duperat V, Ged C, Blouin J M, Richard E, Dabernat S, Moreau-Gaudry F, Bedel A

Abstract excerpt

CRISPR-Cas9 is a promising technology for gene therapy. However, the ON-target genotoxicity of CRISPR-Cas9 nuclease due to DNA double-strand breaks has received little attention and is probably underestimated. Here we report that genome editing targeting globin genes induces megabase-scale losses of heterozygosity (LOH) from the globin CRISPR-Cas9 cut-site to the telomere (5.2 Mb). In established lines,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromosomes, Human, Pair 11
Share to CRISPR-Cas Systems

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Editing

Share to Globins

Share to Hematopoietic Stem Cells

Share to Insulin-Like Growth Factor II

Share to Loss of Heterozygosity

Share to RNA, Long Noncoding

Source

Open in PubMed Open full text

Identifiers

PMID: 34389729
DOI: 10.1038/s41467-021-25190-6
PMCID: PMC8363739

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells.PMID 34389729Read ↗

Single-cell SNP-DNA sequencing precisely maps genotoxic events in CRISPR-edited primary cellsSemantic proximity Read ↗

Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment.Semantic proximity Read ↗

CRISPR RNA-Guided Gene Editing and its Clinical Research Applications in Hematology with Focus on Inherited Germline Predisposition to Hematologic MalignanciesSemantic proximity Read ↗

Detection of deleterious on-target effects after HDR-mediated CRISPR editingSemantic proximity Read ↗

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editingSemantic proximity Read ↗

The CRISPR-Cas System and Clinical Applications of CRISPR-Based Gene Editing in Hematology with a Focus on Inherited Germline Predisposition to Hematologic Malignancies.Semantic proximity Read ↗

Understanding genetic heterogeneity in gene-edited hematopoietic stem cell products.Semantic proximity Read ↗

Simple and reliable detection of CRISPR-induced on-target effects by qgPCR and SNP genotyping.Semantic proximity Read ↗

Human genetic diversity alters therapeutic gene editing off-target outcomesSemantic proximity Read ↗

Chromothripsis as an on-target consequence of CRISPR-Cas9 genome editingSemantic proximity Read ↗

Allele-specific genome editing using CRISPR-Cas9 causes off-target mutations in diploid yeastSemantic proximity Read ↗

Retained chromosomal integrity following CRISPR-Cas9-based mutational correction in human embryos.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.