Article

Human pluripotent reprogramming with CRISPR activators

2017-10-19

Abstract excerpt

CRISPR/Cas9 based gene activation (CRISPRa) is an attractive tool for cellular reprogramming applications due to its high multiplexing capacity and direct targeting of endogenous loci. Here we present the reprogramming of primary human skin fibroblasts into induced pluripotent stem cells (iPSC) using CRISPRa, targeting endogenous OCT4 , SOX2 , KLF4 , MYC and LIN28A promoters. The basal reprogramming efficienc...

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Literature Corpus work: d63be444-7eb3-5b73-aef0-ada8a18ad8ff
DOI: 10.1101/206144

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