Article

Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

2017-03-31

Abstract excerpt

We present a CRISPR/Cas9 genome editing strategy to systematically tag endogenous proteins with fluorescent tags in human inducible pluripotent stem cells. To date we have generated multiple human iPSC lines with GFP tags for 10 proteins representing key cellular structures. The tagged proteins include alpha tubulin, beta actin, desmoplakin, fibrillarin, lamin B1, non-muscle myosin heavy chain IIB, paxillin, Sec61...

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Literature Corpus work: 37f26a9e-7427-52bf-99f9-de4f3c5d4d9c
DOI: 10.1101/123042

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