Article
CRISPR-Cas12a for Highly Efficient and Marker-Free Targeted Integration in Human Pluripotent Stem Cells.
International journal of molecular sciences - 12 Jan 2024
Hammad Ruba, Alzubi Jamal, Rhiel Manuel, Chmielewski Kay O, Mosti Laura, Rositzka Julia, Heugel Marcel, Lawrenz Jan, Pennucci Valentina, Gläser Birgitta, Fischer Judith, Schambach Axel, Moritz Thomas, Lachmann Nico, Cornu Tatjana I, Mussolino Claudio, Schäfer Richard, Cathomen Toni
Abstract excerpt
The CRISPR-Cas12a platform has attracted interest in the genome editing community because the prototypical Acidaminococcus Cas12a generates a staggered DNA double-strand break upon binding to an AT-rich protospacer-adjacent motif (PAM, 5'-TTTV). The broad application of the platform in primary human cells was enabled by the development of an engineered version of the natural Cas12a protein, called Cas12a Ultra....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.