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Article

CRISPR-Cas12a for Highly Efficient and Marker-Free Targeted Integration in Human Pluripotent Stem Cells.

International journal of molecular sciences - 12 Jan 2024

Hammad Ruba, Alzubi Jamal, Rhiel Manuel, Chmielewski Kay O, Mosti Laura, Rositzka Julia, Heugel Marcel, Lawrenz Jan, Pennucci Valentina, Gläser Birgitta, Fischer Judith, Schambach Axel, Moritz Thomas, Lachmann Nico, Cornu Tatjana I, Mussolino Claudio, Schäfer Richard, Cathomen Toni

Abstract excerpt

The CRISPR-Cas12a platform has attracted interest in the genome editing community because the prototypical Acidaminococcus Cas12a generates a staggered DNA double-strand break upon binding to an AT-rich protospacer-adjacent motif (PAM, 5'-TTTV). The broad application of the platform in primary hu...

Topics

Humans
CRISPR-Cas Systems
Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Hematopoietic Stem Cells
Alleles

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 38256061
DOI: 10.3390/ijms25020985
PMCID: PMC10816062

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-38256061-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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