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Article

Activation of pancreatic β-cell genes by multiplex epigenetic CRISPR-editing

2020-07-24

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-based systems for epigenetic editing are promising molecular tools that could be harnessed for directed differentiation of pluripotent stem cells. We used the CRISPR/dCas9-VP160, CRISPR/dCas9-TET1 and CRISPR/dCas9-P300 systems for multiplex epigenetic editing and activation of human beta pancreatic genes ( PDX1, NEUROG3, PAX4 and INS ). The CRISPR/dCas9-P300 system was the most effectiv...

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Literature Corpus work
6850c45a-1573-5a2c-8dbb-30fec3ee3974
DOI
10.1101/2020.07.24.214544
Open publication

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Activation of pancreatic β-cell genes by multiplex epigenetic CRISPR-editingDOI 10.1101/2020.07.24.214544
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