Article

Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells

2024-09-30

Abstract excerpt

Verification of genome editing in human pluripotent stem cells (hPSCs), particularly in silent locus is desirable but challenging because it often requires complex and time-intensive lineage-specific or tissue-specific differentiation to induce their expression. Here, we establish a rapid and effective workflow for the verification of hPSC lines with genome editing in unexpressed genes using CRISPR-mediated transc...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5d4b0ecd-7587-5583-8d1b-b4ce8b4e663d
DOI: 10.1101/2024.09.29.615716

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cellsDOI 10.1101/2024.09.29.615716Read

Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells.Semantic proximity Read

Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions.Semantic proximity Read

Genome-Scale CRISPR Screening Identifies Novel Human Pluripotent Gene NetworksSemantic proximity Read

Multiplexed and Inducible Gene Modulation in Human Pluripotent Stem Cells by CRISPR Interference and ActivationSemantic proximity Read

Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus.Semantic proximity Read

A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells.Semantic proximity Read

Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells.Semantic proximity Read

An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells.Semantic proximity Read

Combining Induced Pluripotent Stem Cells and Genome Editing Technologies for Clinical ApplicationsSemantic proximity Read

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbationSemantic proximity Read

Augmenting and directing long-range CRISPR-mediated activation in human cells.Semantic proximity Read

Enhanced endogenous gene tagging in human induced pluripotent stem cells via AAV6-mediated donor deliverySemantic proximity Read