Article

Comprehensive profiling of transcription factors for reprogramming human astrocytes to neuronal cells through endogenous CRISPR-based gene activation

2025-10-12

Abstract excerpt

<h4>ABSTRACT</h4> Neuronal loss is a hallmark of neurodegeneration and brain injury. Direct reprogramming of astrocytes into neurons has emerged as a promising approach to restore lost neurons. Comprehensive mapping and characterization of candidate astrocyte-to-neuron reprogramming factors is an essential step to realizing the potential of this strategy. Here, we established a CRISPR activation (CRISPRa)-based a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 4702bb06-e69e-5ad3-8b8f-ab1bc4cfd02b
DOI: 10.1101/2025.10.11.681828

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Comprehensive profiling of transcription factors for reprogramming human astrocytes to neuronal cells through endogenous CRISPR-based gene activationDOI 10.1101/2025.10.11.681828Read

Reprogramming of neuronal genome function and phenotype by astrocytesSemantic proximity Read

An Efficient Direct Conversion Strategy to Generate Functional Astrocytes from Human Adult FibroblastsSemantic proximity Read

A Snapshot of Early Transcriptional Changes Accompanying the Pro-Neural Phenotype Switch by NGN2, ASCL1, SOX2, and MSI1 in Human Fibroblasts: An RNA-Seq Study.Semantic proximity Read

CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactionsSemantic proximity Read

CRISPRi screens in human astrocytes elucidate regulators of distinct inflammatory reactive statesSemantic proximity Read

Direct conversion of fibroblasts into functional astrocytes by defined transcription factors.Semantic proximity Read

Dissecting Gene Regulatory Networks Governing Human Cortical Cell FateSemantic proximity Read

Direct reprogramming of human astrocytes into neural stem cells and neuronsSemantic proximity Read

Decoding neuronal diversity by single-cell Convert-seqSemantic proximity Read

GLIS3 is a key regulator of astrocyte differentiation in human neural stem cellsSemantic proximity Read

A CRISPRi/a platform in iPSC-derived microglia uncovers regulators of disease statesSemantic proximity Read

<i>REST</i> and <i>RCOR</i> genes display distinct expression profiles in neurons and astrocytes using 2D and 3D human pluripotent stem cell modelsSemantic proximity Read