Article

Genome-wide CRISPR interference screen identifies long non-coding RNA loci required for differentiation and pluripotency

2021-02-08

Abstract excerpt

<h4>ABSTRACT</h4> Although many long non-coding RNAs (lncRNAs) exhibit lineage-specific expression, the vast majority remain functionally uncharacterized in the context of development. Here, we report the first described human embryonic stem cell (hESC) lines to repress (CRISPRi) or activate (CRISPRa) transcription during differentiation into all three germ layers, facilitating the modulation of lncRNA expression...

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Literature Corpus work: 2ffd8fba-f68d-57bc-b20f-f57b06d4b04b
DOI: 10.1101/2021.02.08.430256

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