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Article

CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing.

Scientific reports - 15 Apr 2016

Chiang Ting-Wei Will, le Sage Carlos, Larrieu Delphine, Demir Mukerrem, Jackson Stephen P

Abstract excerpt

The RNA-guided Cas9 nuclease is being widely employed to engineer the genomes of various cells and organisms. Despite the efficient mutagenesis induced by Cas9, off-target effects have raised concerns over the system's specificity. Recently a "double-nicking" strategy using catalytic mutant Cas9(D10A) nickase has been developed to minimise off-target effects. Here, we describe a Cas9(D10A)-based screening...

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Share to Deoxyribonuclease I
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 27079678
DOI: 10.1038/srep24356
PMCID: PMC4832145

Genes

MDC1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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