Article

Kozak sequence libraries for characterizing transgenes across expression levels

2025-04-30

Abstract excerpt

Typical mammalian overexpression systems test protein sequence variants with little control over expression levels and steady-state protein abundances, hindering interpretations of how protein sequence and expression converge to yield phenotypic outcomes. We explored the translation initiation sequence, commonly referred to as the Kozak sequence, as a means to modulate protein steady-state abundance and cellular f...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d1dfc8c6-babe-511d-8cc6-0eeb6e759091
DOI: 10.1101/2025.04.28.651141

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Kozak sequence libraries for characterizing transgenes across expression levelsDOI 10.1101/2025.04.28.651141Read

Splice-Aware Optimization Prevents Pervasive Missplicing of Natural and Synthetic cDNAsSemantic proximity Read

5’Untranslated regions provide a versatile toolkit for tunable exogenous protein expressionSemantic proximity Read

A single-nucleotide change in the Kozak sequence enhances protein expressionSemantic proximity Read

Gene library deep sequencing for protein super-family profilingSemantic proximity Read

Human cellular homeostasis buffers <i>trans</i> -acting translational effects of heterologous gene expression with very different codon usage biasSemantic proximity Read

ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomesSemantic proximity Read

Combinatorial CRISPR screening reveals functional buffering in autophagySemantic proximity Read

Transposon-Display of AI-designed binders enables manipulation of the proteome in human cellsSemantic proximity Read

Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency.Semantic proximity Read

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scaleSemantic proximity Read

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput screensSemantic proximity Read

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scale.Semantic proximity Read