Article

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scale

2023-02-12

Abstract excerpt

<h4>Motivation</h4> Analysis of allele-specific expression is strongly affected by the technical noise present in RNA-seq experiments. Previously, we showed that technical replicates can be used for precise estimates of this noise, and we provided a tool for correction of technical noise in allele-specific expression analysis. This approach is very accurate but costly due to the need for two or more replicates of...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0a201180-73a4-5198-9043-81e0fb52ad2d
DOI: 10.1101/2023.02.11.528027

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scaleDOI 10.1101/2023.02.11.528027Read

Foreign RNA spike-ins enable accurate allele-specific expression analysis at scale.Semantic proximity Read

Synthesizing human-specific spike-in standards for RNA-seq experiments and assessing their technical performanceSemantic proximity Read

Normalization of human RNA-seq experiments using chimpanzee RNA as a spike-in standard.Semantic proximity Read

Beyond library size: a field guide to NGS normalizationSemantic proximity Read

The effect of background noise and its removal on the analysis of single-cell expression data.Semantic proximity Read

Evaluating Methods for Differential Gene Expression And Alternative Splicing Using Internal Synthetic ControlsSemantic proximity Read

CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomicsSemantic proximity Read

Transcriptome Universal Single-isoform COntrol: A Framework for Evaluating Transcriptome reconstruction QualitySemantic proximity Read

High sensitivity single cell RNA sequencing with split pool barcodingSemantic proximity Read

Full-length mRNA sequencing reveals principles of poly(A) tail length controlSemantic proximity Read

Droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencingSemantic proximity Read

Replicate sequencing libraries are important for quantification of allelic imbalance.Semantic proximity Read