Article

One-step generation of auxin-inducible degron cells with high-efficiency homozygous tagging

2023-03-27

Abstract excerpt

Auxin-inducible degron (AID) technology is powerful for chemogenetic control of proteolysis. However, generation of human cell lines to deplete endogenous proteins with AID remains challenging. Typically, homozygous degron-tagging efficiency is low and overexpression of an auxin receptor requires additional engineering steps. Here, we establish a one-step genome editing procedure with high-efficiency homozygous ta...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f0671e28-1475-55ae-88e0-23f154467bb5
DOI: 10.1101/2023.03.26.534268

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

One-step generation of auxin-inducible degron cells with high-efficiency homozygous taggingDOI 10.1101/2023.03.26.534268Read

HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cellsSemantic proximity Read

Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cellsSemantic proximity Read

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice.Semantic proximity Read

Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinoleSemantic proximity Read

Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology DonorsSemantic proximity Read

Comparative analysis and directed protein evolution yield an improved degron technology with minimal basal degradation, rapid inducible depletion, and faster recovery of target proteins.Semantic proximity Read

The mIAA7 degron improves auxin-mediated degradation in <i>C. elegans</i>Semantic proximity Read

A super sensitive auxin-inducible degron system with an engineered auxin-TIR1 pairSemantic proximity Read

Deciphering acquired resistance mechanisms to sustained auxin-inducible protein degradation in cells and miceSemantic proximity Read

Expanding the <i>Caenorhabditis elegans</i> auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editingSemantic proximity Read

A bulk cell heterozygous knock-in strategy for targeted protein degradationSemantic proximity Read

Efficacy of auxin-inducible protein degradation in <i>C. elegans</i> tissues using different auxins and TIR1-expressing strainsSemantic proximity Read