Article

5’Untranslated regions provide a versatile toolkit for tunable exogenous protein expression

2026-01-19

Abstract excerpt

<h4>ABSTRACT</h4> Transient transfection is widely used for protein expression in heterologous systems, yet uncontrolled overexpression frequently introduces artifacts that confound functional analyses. Although stable cell lines can mitigate these issues, generating lines for multiple constructs or variants is often impractical. Common alternatives, such as DNA titration, altered transfection conditions, or prom...

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Literature Corpus work: c4d7b005-de1e-54e0-afb2-8ee4506ae95f
DOI: 10.64898/2026.01.19.700389

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