Article

A single-nucleotide change in the Kozak sequence enhances protein expression

2026-05-21

Abstract excerpt

In vitro -transcribed messenger RNA (IVT mRNA) has emerged as a versatile protein expression platform with broad clinical potential. Current optimization strategies for IVT mRNA focus on untranslated regions (UTRs), mRNA stability, and codon usage, often guided by massively parallel screening and machine learning approaches. In contrast, the Kozak sequence, a key determinant of translation initiation, is often inc...

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Literature Corpus work: e422ac56-5f2b-5c25-8237-e3ca210e871f
DOI: 10.64898/2026.05.20.726450

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