Article

Gene library deep sequencing for protein super-family profiling

2025-10-21

Abstract excerpt

Unravelling protein function at scale remains challenging. Gain-of-function (GOF) screens harnessing large open reading frame (ORF) libraries enable systematic functional annotations of cell behavior drivers and drug targets. Currently, few sequencing methods are established to analyze existing ORF libraries and facilitate future library generation. Here, we developed a barcode-free deep sequencing method termed p...

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Identifiers and source

Literature Corpus work: 1331356d-5ede-5161-94f5-a7489ebb03ce
DOI: 10.1101/2025.10.20.682913

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