Article

Splice-Aware Optimization Prevents Pervasive Missplicing of Natural and Synthetic cDNAs

2026-05-25

Abstract excerpt

<h4>ABSTRACT</h4> Heterologous gene expression is widely used across biology and medicine, and often relies on codon optimization to increase protein yields. Here we uncover missplicing as a common and largely unrecognized failure mode of heterologous expression. Using systematically designed libraries comprising over 5,000 synthetic reporter genes and natural human cDNAs, we find that the majority of gene varian...

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Literature Corpus work: 65c85962-4c33-55c1-aad9-9cfcbdf209e9
DOI: 10.64898/2026.05.25.727620

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