Article

sc-SPLASH provides ultra-efficient reference-free discovery in barcoded single-cell sequencing

2024-12-24

Abstract excerpt

Typical high-throughput single-cell RNA-sequencing (scRNA-seq) analyses are primarily conducted by (pseudo)alignment, through the lens of annotated gene models, and aimed at detecting differential gene expression. This misses diversity generated by other mechanisms that diversify the transcriptome such as splicing and V(D)J recombination, and is blind to sequences missing from imperfect reference genomes. Here, we...

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Literature Corpus work: d15aee27-823f-5b35-9bc7-5727764a290d
DOI: 10.1101/2024.12.24.630263

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