A FRET based biosensor for measuring Gα13 activation in single cells

Abstract excerpt

Förster Resonance Energy Transfer (FRET) provides a way to directly observe the activation of heterotrimeric G-proteins by G-protein coupled receptors (GPCRs). To this end, FRET based biosensors are made, employing heterotrimeric G-protein subunits tagged with fluorescent proteins. These FRET based biosensors complement existing, indirect, ways to observe GPCR activation. Here we report on the insertion of mTurquo...

Topics

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• Amino Acid Motifs
• Biosensing Techniques
• GTP-Binding Protein alpha Subunits, Gq-G11
• Guanine Nucleotide Exchange Factors
• Guanosine Triphosphate
• Heterotrimeric GTP-Binding Proteins
• Receptors, G-Protein-Coupled
• Urinary Bladder Neoplasms

Identifiers and source

Literature Corpus work

cfe83ac2-bd35-5350-923f-e2a10874d6a7

DOI

10.1101/220632