Article

Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors

2021-02-06

Abstract excerpt

Heterotrimeric G proteins constitute the primary transducers of G protein-coupled receptor (GPCR) signaling. Besides mediating ligand-induced GPCR activation, G proteins transduce basal levels of activity in various physiological and pathophysiological settings evoked by constitutively active, native GPCRs or disease-related receptor mutants. Several generations of optical biosensors were developed and optimized t...

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Literature Corpus work: 07d8eb7d-00bd-5bd1-b8e1-0322fa91b995
DOI: 10.1101/2021.02.05.429900

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