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Article

Engineered interfaces in Rac1 and Cdc42 biosensors enhance sensitivity and reduce cell perturbation.

Molecular biology of the cell - 1 Apr 2026

Marston Daniel J, O'Shaughnessy Ellen C, Jacobs Timothy M, Huh Jaewon, Tsygankov Denis, Choi Mingyu, Ma Xiao, Azoitei Mihai L, Danuser Gaudenz, Kuhlman Brian, Hahn Klaus M

Abstract excerpt

Fluorescent biosensors are a valuable means to report the spatiotemporal dynamics of protein activities in live cells and animals. However, biosensors affect the activities they are reporting. This can be ameliorated by increasing sensitivity, using lower biosensor concentrations, or by choosing designs that minimize undesirable interactions. For biosensors in which fluorescent components interact to produce...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41705965
DOI: 10.1091/mbc.E25-10-0494
PMCID: PMC13041527

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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