Article

FRET and LRET Biosensors for Cell-based Imaging and Screening of Rac1 Activation

2021-06-26

Abstract excerpt

<h4>ABSTRACT</h4> Rac1 is a key regulator of several cell signaling pathways and dysregulated Rac1 activation has been implicated in cancer. Genetically encoded Förster resonance energy transfer (FRET) biosensors with enhanced dynamic range enabled live cell fluorescence imaging of Rac1 activity and a cell lysate-based assay of Rac1 inhibition in 96-well plates. We prepared HEK293 cell lines that stably expressed...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 94c2b33d-4a6e-5cfa-8b34-423365e51651
DOI: 10.1101/2021.06.25.449998

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

FRET and LRET Biosensors for Cell-based Imaging and Screening of Rac1 ActivationDOI 10.1101/2021.06.25.449998Read

A method to measure the interaction of Rac/Cdc42 with their binding partners using fluorescence resonance energy transfer between mutants of green fluorescent protein.Semantic proximity Read

Integration of FRET and sequencing to engineer kinase biosensors from mammalian cell librariesSemantic proximity Read

Combining FRET and super-resolution microscopy reveals kinase activation and mitochondrial activity at the nanoscaleSemantic proximity Read

Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensorsSemantic proximity Read

EGFR signaling and pharmacology in oncology revealed with innovative BRET-based biosensorsSemantic proximity Read

A rapid fluorogenic GPCR-β-arrestin interaction assay.Semantic proximity Read

BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions.Semantic proximity Read

Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells.Semantic proximity Read

In vivo monitoring of the recruitment and activation of AP-1 by Arf1.Semantic proximity Read

A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition.Semantic proximity Read

Mimicking transient activation of protein kinases in living cells.Semantic proximity Read

BRED: bioluminescence energy transfer to dye for monitoring ceramide trafficking in cellSemantic proximity Read