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Article

Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design.

Cell - 6 Aug 2020

Maziarz Marcin, Park Jong-Chan, Leyme Anthony, Marivin Arthur, Garcia-Lopez Alberto, Patel Prachi P, Garcia-Marcos Mikel

Abstract excerpt

Heterotrimeric G-proteins (Gαβγ) are the main transducers of signals from GPCRs, mediating the action of countless natural stimuli and therapeutic agents. However, there are currently no robust approaches to directly measure the activity of endogenous G-proteins in cells. Here, we describe a suite of optical biosensors that detect endogenous active G-proteins with sub-second resolution in live cells. Using a...

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Identifiers

PMID: 32634377
DOI: 10.1016/j.cell.2020.06.020
PMCID: PMC7415655

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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