Article

Evaluation and Design of Genome-wide CRISPR/Cas9 Knockout Screens

2017-03-16

Abstract excerpt

The adaptation of CRISPR/Cas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs), targeting human protein-coding genes and encoded in viral vectors, have been used to systematically create gene knockouts in a variety of human cancer and immortalized cell lines, in an effort to identify whether these knockouts cause cellular fitne...

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Literature Corpus work: 19c14cb4-dfdf-55a5-8d5d-fd8cbd493c50
DOI: 10.1101/117341

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