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Article

Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9.

Fly - 2 Jan 2017

Lamb Abigail M, Walker Elizabeth A, Wittkopp Patricia J

Abstract excerpt

Genome editing via the CRISPR/Cas9 RNA-guided nuclease system has opened up exciting possibilities for genetic analysis. However, technical challenges associated with homology-directed repair have proven to be roadblocks for producing changes in the absence of unwanted, secondary mutations commonly known as "scars." To address these issues, we developed a 2-stage, marker-assisted strategy to facilitate precise,...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 27494619
DOI: 10.1080/19336934.2016.1220463
PMCID: PMC5354236

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Source receipt: pubmed, retrieved 7 Sept 2026.

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