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Programmable Cleavage of Double-stranded DNA by Combined Action of Argonaute CbAgo from <i>Clostridium butyricum</i> and Nuclease Deficient RecBC Helicase from <i>E.coli</i>

2021-07-01

Abstract excerpt

<h4>ABSTRACT</h4> Prokaryotic Argonautes (pAgos) use small nucleic acids as specificity guides to cleave single-stranded DNA at complementary sequences. DNA targeting function of pAgos creates attractive opportunities for DNA manipulations that require programmable DNA cleavage. Discovery of mesophilic Argonautes active at physiological temperature places pAgos closer to their possible application for genome edit...

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Literature Corpus work
c1437c86-933a-5ff0-a8c6-e1eefc3593eb
DOI
10.1101/2021.07.01.450725
Open publication

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Programmable Cleavage of Double-stranded DNA by Combined Action of Argonaute CbAgo from <i>Clostridium butyricum</i> and Nuclease Deficient RecBC Helicase from <i>E.coli</i>DOI 10.1101/2021.07.01.450725
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