Article

Sequence-Specific Recognition of Double-Stranded Nucleic Acids by λ Exonuclease-Phosphorylated DNA Mediated Target Interrogation

2023-11-07

Abstract excerpt

<title>Abstract</title> <p>The CRISPR-Cas system, guided by an RNA molecule, cleaves double-stranded DNA targets in proximity to a protospacer adjacent motif (PAM), enabling the applications in genetic editing/interference and molecular diagnostics<sup>1–3</sup>. However, the reliance on PAM-dependent recognition and cleavage has limited the range of targetable sequences and led to undesired off-target effects, r...

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Literature Corpus work: 484b6c0e-9fcf-5c3c-863a-9105c31c0472
DOI: 10.21203/rs.3.rs-3511188/v1

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