Article
DNA targeting by Clostridium cellulolyticum CRISPR-Cas9 Type II-C system.
Nucleic acids research - 28 Feb 2020
Fedorova Iana, Arseniev Anatolii, Selkova Polina, Pobegalov Georgii, Goryanin Ignatiy, Vasileva Aleksandra, Musharova Olga, Abramova Marina, Kazalov Maksim, Zyubko Tatyana, Artamonova Tatyana, Artamonova Daria, Shmakov Sergey, Khodorkovskii Mikhail, Severinov Konstantin
Abstract excerpt
Type II CRISPR-Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a relatively big size, imperfect specificity and restriction to DNA targets flanked by an NGG PAM sequence. Cas9 orthologs from other bacterial species may provide a rich...
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