Article

Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnostics

2026-06-01

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR–Cas12a underlies powerful genome-editing and nucleic-acid detection technologies, yet its performance is limited by inefficient target engagement and low catalytic turnover, particularly at low target abundance and elevated temperatures. Here, we report a modular protein-engineering strategy to enhance Cas12a trans-activity by fusing the hyperthermophilic DNA-binding protein Sso7d to the...

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Literature Corpus work: c9cde72b-6944-5c28-b2a3-c9eb0b67e0e1
DOI: 10.64898/2026.05.30.729025

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