Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

Abstract excerpt

CRISPR/Cas9 system is а powerful gene editing tool based on the RNA-guided cleavage of target DNA. The Cas9 activity can be modulated by proteins involved in DNA damage signalling and repair due to their interaction with double- and single-strand breaks (DSB and SSB, respectively) generated by wild-type Cas9 or Cas9 nickases. Here we address the interplay between Streptococcus pyogenes Cas9 and key DNA repair fac...

Topics

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• Base Sequence
• Binding Sites
• Deoxyribonuclease I
• DNA
• Genome, Human
• HeLa Cells
• Models, Genetic
• Mutation

Identifiers and source

Literature Corpus work

1393a56e-8c39-5d22-9457-8c219846e7d3

DOI

10.1101/2023.09.05.556296