Article

The stepwise endonuclease activity of a thermophilic Argonaute protein

2019-10-29

Abstract excerpt

Thermophilic Argonaute proteins (Agos) can function as endonucleases via specific guide-target base-pairing cleavage for host defense. The ability to cleave target DNA sequences at any arbitrary sites endows them with reprogramed DNA capacity. Here, we identify that an Ago from the hyperthermophilic archaeon Pyrococcus furiosus ( Pf Ago) shows a stepwise endonuclease activity, which is demonstrated by the double...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 71e6df7e-51e7-538a-a962-2759b4bab428
DOI: 10.1101/821280

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

The stepwise endonuclease activity of a thermophilic Argonaute proteinDOI 10.1101/821280Read

Advancements in Pathogen Detection: Argonaute-Based Nucleic Acid Detection TechnologySemantic proximity Read

Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations.Semantic proximity Read

DNA interference by a mesophilic Argonaute protein, CbcAgoSemantic proximity Read

Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.Semantic proximity Read

RNA-guided RNA silencing by an Asgard archaeal ArgonauteSemantic proximity Read

Highly specific enrichment of rare nucleic acids using <i>Thermus thermophilus</i> ArgonauteSemantic proximity Read

Repurpose Type I-A CRISPR-Cas3 for a Robust HPV DiagnosisSemantic proximity Read

Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)Semantic proximity Read

The Role of Nucleases and Nucleic Acid Editing Enzymes in the Regulation of Self-Nucleic Acid SensingSemantic proximity Read

Programmable RNA-guided endonucleases are widespread in eukaryotes and their virusesSemantic proximity Read

Programmable Cleavage of Double-stranded DNA by Combined Action of Argonaute CbAgo from <i>Clostridium butyricum</i> and Nuclease Deficient RecBC Helicase from <i>E.coli</i>Semantic proximity Read

Catalytic rewiring of RuvC-II catalytic site activates <i>trans</i> -cleavage in Fanzor2Semantic proximity Read