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Repurposing CRISPR-Cas12b for mammalian genome engineering

14 Nov 2018

Abstract excerpt

Abstract The prokaryotic CRISPR-Cas adaptive immune systems provide valuable resources to develop genome editing tools, such as CRISPR-Cas9 and CRISPR-Cas12a/Cpf1. Recently, CRISPR-Cas12b/C2c1, a distinct type V-B system, has been characterized as a dual-RNA-guided DNA endonuclease system. Though being active in vitro, its cleavage activity at endogenous genome remains to be explored. Furthermore, the optimal...

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PMID: 30510770
DOI: 10.1038/s41421-018-0069-3

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