Article

CRISPR-Cas12a REC2 – NUC interactions drive target-strand cleavage and constrain trans cleavage

2025-03-25

Abstract excerpt

CRISPR-Cas12a effects RNA-guided cleavage of dsDNA in cis , after which it remains catalytically active and non-specifically cleaves ssDNA in trans . Native host-defence by Cas12a employs cis cleavage, which can be repurposed for the genome editing of other organisms, and trans cleavage can be used for in vitro DNA detection. Cas12a orthologues have high structural similarity and a conserved mechanism of DNA...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 09e5ff5d-5cad-58ec-81b7-fea64048b42d
DOI: 10.1101/2025.03.23.644851

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas12a REC2 – NUC interactions drive target-strand cleavage and constrain trans cleavageDOI 10.1101/2025.03.23.644851Read

Nuc domain electrostatics drive the trans cleavage activity of CRISPR-Cas12a.Semantic proximity Read

Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnosticsSemantic proximity Read

Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell eliminationSemantic proximity Read

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12aSemantic proximity Read

Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavageSemantic proximity Read

Structural principles of CRISPR-Cas enzymes used in nucleic acid detectionSemantic proximity Read

A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical SamplesSemantic proximity Read

Harnessing Non-Canonical crRNAs to Improve Functionality of Cas12a OrthologsSemantic proximity Read

Massively parallel kinetic profiling of natural and engineered CRISPR nucleasesSemantic proximity Read

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.Semantic proximity Read

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavageSemantic proximity Read

A naturally DNase-free CRISPR-Cas12c enzyme silences gene expressionSemantic proximity Read